Journal: Poultry Science

Article Title: Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7

doi: 10.1016/j.psj.2020.12.037

Figure Lengend Snippet: Reactivity assessment of chicken IL7 mAb to BL21-derived or Chinese hamster ovary cells-derived recombinant chIL7 by Western blot. One μg per lane (1 and 7) BL21-derived (A) or Chinese hamster ovary cells-derived (C) rchIL7 were resolved in 12% SDS-PAGE and probed with the following chIL7 mAb: 2 and 8, 1B7; 3 and 9, 3D3; 4 and 10, 1H6; 5 and 11, 10H3; and 6 and 12, 4F12 (B and D). Abbreviations: mAb, monoclonal antibodies; SDS-PAGE, sodium dodecyl sulfate/polyacrylamide.

Article Snippet: Human and mouse IL7 recombinant proteins (PeproTech, NJ) were used as specificity controls in chIL7 sandwich ELISA standardization.

Techniques: Derivative Assay, Recombinant, Western Blot, SDS Page, Bioprocessing

Journal: Poultry Science

Article Title: Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7

doi: 10.1016/j.psj.2020.12.037

Figure Lengend Snippet: Pairing chIL7 mAb, sandwich ELISA, standard curve, specificity and detection of chIL7 levels in chicken unchallenged and challenge with Eimeria acervulina. (A) ChIL7 mAb pairing by sandwich ELISA. Plot of chIL7 mAb binding with different affinities to BL-21-derived rchIL7 as assayed by sandwich ELISA. (B) ChIL7 sandwich ELISA standard curve using a mAb pair, 1B7 and biotinylated 10H3. (C) ChIL7 sandwich ELISA specificity. ∗∗∗ P < 0.001 as compared with the blank. (D) ChIL7 levels in bloodstream of chickens challenged by E. acervulina . Fourteen-day-old chickens were challenged with E. acervulina (1 x 10 4 sporulated oocysts), blood was collected at 9 d after challenge, and chIL7 protein levels were measured by sandwich ELISA. Samples were run in triplicates. ∗ P < 0.05 as compared with the unchallenged group. Data are represented mean ± SD of triplicate from 3 independent experiments. Abbreviations: ChIL7, chicken IL7; mAb, mAb, monoclonal antibodies.

Article Snippet: Human and mouse IL7 recombinant proteins (PeproTech, NJ) were used as specificity controls in chIL7 sandwich ELISA standardization.

Techniques: Sandwich ELISA, Binding Assay, Derivative Assay, Bioprocessing

Journal: Cell reports

Article Title: HSC-independent definitive hematopoiesis persists into adult life

doi: 10.1016/j.celrep.2023.112239

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant mouse IL-7 , PeproTech , 217-17.

Techniques: Recombinant, Software

Journal:

Article Title: Epstein-Barr Virus LMP2A Interferes with Global Transcription Factor Regulation When Expressed during B-Lymphocyte Development

doi: 10.1128/JVI.77.1.105-114.2003

Figure Lengend Snippet: LMP2A transgenic mice and B-lymphocyte development. (A) Upper panel, bone marrow (BM) (left) and splenic (right) B cells were purified from wild-type (WT) and LMP2A transgenic mice. Cells were stained with antibodies to CD19, B220, CD43, and IgM to detect cell surface expression. The numbers indicate the percentage of cells positive for expression. Lower panel, CD19+ bone marrow B cells were purified in methylcellulose cultures containing IL-7, and splenic B cells were isolated by using CD19-coated magnetic beads as described in Materials and Methods. (B) Stages of B-cell development in the bone marrow, beginning from common lymphoid progenitor (CLP) cells and progressing to immature B cells, which exit the bone marrow and colonize peripheral lymphoid organs. The transition from the pro-B stage to the pre-B stage of development requires both E2A and EBF transcription factors. Pax-5 is required for B-cell commitment and transition through the pre-B stage. The activity of E2A is inhibited by Id and SCL proteins (for reviews, see references 27, 29, and 61).

Article Snippet: Red blood cells were lysed in 155 mM ammonium chloride, and 2 × 10 6 cells were placed in 3 ml of Methocult M3630 methylcellulose cultures containing 10 ng of recombinant mouse IL-7 (Stemcell Technologies, Vancouver, British Colombia, Canada) per ml.

Techniques: Transgenic Assay, Purification, Staining, Expressing, Isolation, Magnetic Beads, Activity Assay