Journal:

Article Title: Epstein-Barr Virus LMP2A Interferes with Global Transcription Factor Regulation When Expressed during B-Lymphocyte Development

doi: 10.1128/JVI.77.1.105-114.2003

Figure Lengend Snippet: LMP2A transgenic mice and B-lymphocyte development. (A) Upper panel, bone marrow (BM) (left) and splenic (right) B cells were purified from wild-type (WT) and LMP2A transgenic mice. Cells were stained with antibodies to CD19, B220, CD43, and IgM to detect cell surface expression. The numbers indicate the percentage of cells positive for expression. Lower panel, CD19+ bone marrow B cells were purified in methylcellulose cultures containing IL-7, and splenic B cells were isolated by using CD19-coated magnetic beads as described in Materials and Methods. (B) Stages of B-cell development in the bone marrow, beginning from common lymphoid progenitor (CLP) cells and progressing to immature B cells, which exit the bone marrow and colonize peripheral lymphoid organs. The transition from the pro-B stage to the pre-B stage of development requires both E2A and EBF transcription factors. Pax-5 is required for B-cell commitment and transition through the pre-B stage. The activity of E2A is inhibited by Id and SCL proteins (for reviews, see references 27, 29, and 61).

Article Snippet: Red blood cells were lysed in 155 mM ammonium chloride, and 2 × 10 6 cells were placed in 3 ml of Methocult M3630 methylcellulose cultures containing 10 ng of recombinant mouse IL-7 (Stemcell Technologies, Vancouver, British Colombia, Canada) per ml.

Techniques: Transgenic Assay, Purification, Staining, Expressing, Isolation, Magnetic Beads, Activity Assay